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5. Two months after operation, the SEM observation of the dentin barrier formed in the amputated plane showed tissue fusion with primary dentin and smooth surfaces continuously from the peripheries to the center of the pulp. The TEM observation of this region showed highly active odontoblasts. 6. In this study, dentin bond fatigue resistance and interfacial science characteristics of universal adhesives through etch-and-rinse and self-etch modes were investigated. Resin composite was bonded to human dentin with four universal adhesives, namely, Adhese Universal, All-Bond Universal, G-Premio Bond, and Scotchbond Universal Adhesive. The results were as follows: 1. The dentin bond strengths of the single-step self-etch systems decreased with increase of RH, and significant decreases in bond strengths were observed except for G-Bond and One-up Bond F Plus. 2. According to the fracture mode after the test, adhesive failure tended to increase with increase of RH. Here, we investigated the incremental growth lines in mouse molar dentin using tetracycline as the growth marker. The incremental growth lines were observed to be generated at approximately 8-hr intervals in wild-type mice housed under 12:12 hr light-dark conditions.